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Differences and associations of metabolic and vitamin D status among patients with and without sub-clinical hypothyroid dysfunction
© Aljohani et al.; licensee BioMed Central Ltd. 2013
Received: 18 June 2013
Accepted: 20 August 2013
Published: 20 August 2013
Sub-clinical hypothyroid dysfunction, a relatively understudied disorder in the Kingdom of Saudi Arabia (KSA), has significant clinical implications if not properly monitored. Also from KSA, more than 50% of the population suffer from hypovitaminosis D (<50 nmol/l). In this cross-sectional case-control study, we described the differences and associations in the metabolic patterns of adult Saudis with and without hypothyroid dysfunction in relation to their vitamin D status, PTH, calcium and lipid profile.
A total of 94 consenting adult Saudis [52 controls (without subclinical hypothyroidism), 42 cases (previously diagnosed subjects)] were included in this cross-sectional study. Anthropometrics were obtained and fasting blood samples were taken for ascertaining lipid and thyroid profile, as well as measuring PTH, 25(OH) vitamin D and calcium.
Cases had a significantly higher body mass index than the controls (p < 0.001). Circulating triglycerides was also significantly higher in cases than the controls (p = 0.001). A significant positive association between HDL-cholesterol and PTH (R = 0.56; p = 0.001), as well as a negative and modestly significant negative association between LDL-cholesterol and PTH (R = - 20.0; p = 0.04) were observed. FT3 was inversely associated with circulating 25 (OH) vitamin D (R = -0.25; p = 0.01).
Patients with hypothyroid dysfunction possess several cardiometabolic risk factors that include obesity and dyslipidemia. The association between PTH and cholesterol levels as well as the inverse association between vitamin D status and FT3 needs to be reassessed prospectively on a larger scale to confirm these findings.
Thyroid diseases are one of the more commonly encountered endocrine disorders in the Kingdom of Saudi Arabia (KSA), next to diabetes mellitus (DM) . While current demographics point to less alarming prevalence of thyroid dysfunction, certain populations such as children with Down syndrome appear to manifest conditions related to thyroid dysfunction . In the Middle East in general, thyroid dysfunction, specifically subclinical hypothyroid dysfunction, is a relatively understudied field as compared to other metabolic disorders such as obesity and insulin resistance. In a recent study, Bahammam and colleagues observed that subclinical hypothyroid dysfunction was prevalent among Saudi patients suffering from obstructive sleep apnea (OSA), with an estimated 1 out of 10 OSA patients harboring the condition . Granted that hypothyroid dysfunction is not as endemic as the previously mentioned disorders, it nevertheless deserves attention as it can lead to serious repercussions if left ignored. Several studies point to an association between thyroid diseases, particularly Grave’s disease and vitamin D status in several ethnic groups, but none if not limited in the Middle East [4–6]. In this cross-sectional case-control study, we describe the differences and associations in the metabolic patterns of adult Saudis with and without subclinical hypothyroid dysfunction in relation to lipid and calcium metabolism in an attempt to stimulate further clinical investigations with respect to this understudied disorder.
A total of 94 consenting adult Saudis [52 control (6 males; 46 females), 42 cases (3 males, 39 females)] were included in this cross-sectional study. All subjects were recruited at the Endocrinology Unit of King Fahad Medical City, Riyadh Saudi Arabia. Adult Saudis aged 20-50, who are known cases of subclinical hypothyroid dysfunction based on previous clinical assessment (elevated TSH with normal FT4 levels), medications taken and laboratory tests with no complications were included. Control subjects were those who tested negative for subclinical hypothyroid dysfunction with no history of thyroid medications. Non-Saudis and pregnant women as well as children were excluded. Written and informed consent were secured prior to inclusion. Ethical approval was obtained from the Institutional Review Board of King Fahad Medical City, Riyadh, KSA.
Anthropometric parameters were obtained while the subject was standing erect and barefoot. Height and weight were determined using standardized conventional methods. Body mass index (BMI) was calculated using the formula: weight in kilograms (kg) divided by height in squared meters (m2).
Blood (≈ 10cc) was withdrawn after an overnight fast (> 10 hours). Serum calcium was measured using standard analytical techniques (Konelab, Finland). Fasting blood lipids which included triglycerides, total, HDL- and LDL-cholesterol as well as calcium were measured routinely using a chemical analyzer (Konelab, Finland). Serum TSH, FT3, and FT4 were estimated using commercially available kits by Roche Elecsys Modular Analytics Cobas e411 utilizing electrochemiluminescence immunoassay (Roche Diagnostics, Mannheim, Germany). TSH upper normal limit for males is 3.69 μIU/ml and 3.94 μIU/ml for females. Intact PTH and 25(OH)D were measured by specific ELISAs in accordance with the instructions provided by the manufacturer (IDS, Tyne & Wear, UK). 25(OH) vitamin D has been the preferred metabolite for the measurement of vitamin D status instead of 1, 25(OH)D, and remains the basis for the diagnosis of vitamin D deficiency . The inter- and intra-assay variabilities were 5.8% and 3.4% respectively for the intact PTH ELISA, 5.3% and 4.6% respectively for the 25(OH)D ELISA. Anti-thyroid peroxidase (TPO) antibodies (Ab) were also measured using commercially available ELISA kits (Bio-Line S.A, Brussels, Belgium) with a sensitivity of 1.4 U/ml (intra- assay variability 6.9%; inter-assay variability 13.4%).
Data was analyzed using the Statistical Package for the Social Sciences (SPSS) version 16.5 (Chicago, IL, USA). Frequencies were presented as N and continuous variables if normal were presented as mean ± standard deviation and median (inter-quartile range) for variables with non-normal distribution. For group comparisons (controls versus cases), independent T-test if normally distributed continuous variables, and Mann-Whitney U-test for variables with non-Gaussian distribution were utilized. Significance set at p < 0.05.
Differences in metabolic characteristics of controls versus cases
36.1 ± 8.1
35.5 ± 10.1
Body Mass Index (kg/m2)
27.8 ± 3.9
32.3 ± 6.8
1.2 ± 0.26
1.9 ± 0.46
Total Cholesterol (mmol/l)
5.1 ± 0.80
5.2 ± 1.0
3.9 ± 0.88
3.9 ± 0.87
0.90 ± 0.37
0.88 ± 0.30
119.5 ± 35.9
97.6 ± 38.5
2.4 (1.6, 3.3)
3.7 (1.5, 7.6)
6.0 ± 1.3
4.8 ± 1.2
17.2 ± 1.3
17.5 ± 1.2
TPOAb (U/ml) #
17.7 (14.1, 25.0)
164.9 (15.8, 582.9)
5.3 ± 1.5
8.0 ± 1.2
33.8 ± 5.4
36.5 ± 6.8
Corrected Ca (mmol/l)
2.4 ± 0.23
2.1 ± 0.18
2.5 ± 0.19
2.1 ± 0.45
25 (OH) Vitamin D (nmol/l)
18.3 ± 1.5
37.3 ± 1.9
Correlation coefficients (R) of Lipids to thyroid function tests, PTH and vitamin D adjusted for age and BMI
Total Cholesterol (mmol/l)
The main finding of this study is that aside from the conventional and expected differences in the expression of thyroid tests, including iodine and TPOab, patients with hypothyroid dysfunction also exhibited obesity and elevated levels of triglycerides as compared to controls. How subclinical hypothyroid dysfunction influences weight gain can be explained via the peripheral effects of thyroid hormones and their local regulation of central nervous system (CNS) in the physiologic regulation of appetite that is independent of their conventional role in basal energy expenditure as well as regulation of resting energy expenditure [8, 9]. With respect to obesity per se, it has been found out that visceral adipose tissue has a direct effect in TSH levels among obese, and this is independent of insulin resistance . Regarding triglycerides, our results were in accordance with the study of Wanjia and colleagues, who also observed elevated TSH levels among patients with hypertriglyceridemia . The exact mechanisms accountable for the effects of thyroid function with respect to lipid profile are unclear. Nevertheless it is almost established that TSH receptors are present in tissues other than the thyroid gland which include kidneys, bone marrow and adipose tissue, and that variations ion TSH levels among euthyroid patients are partially explained by lipid components and hypercholesterolemia which are independent of thyroid hormones [12, 13]. Furthermore, there are several factors not accounted for in this study that may influence thyroid function and lipid profile, including smoking and insulin resistance , as well as obesity and abnormalities in glucose tolerance.
One striking and unexpected finding in this study is the significantly higher mean 25(OH) vitamin D levels among patients with subclinical hypothyroid dysfunction than controls despite the presence of established risk factors for vitamin D deficiency including obesity and hypetriglyceridemia [15–17]. Calcium levels while also significantly different, still fall within normal range, although the possibility of other causes such as malabsorption or decreased dietary calcium intake in some subjects cannot be ruled out. Furthermore, PTH levels were higher, although not significant in the case group than controls. These differences this can be attributed to several confounders unaccounted for in the study which include differences in dietary calcium and vitamin D intake, undocumented comorbidities such as malabsorption and existing renal disorders which were not evident at the time of selection, as well as sun exposure, and needs further investigation. Nevertheless, while the vitamin D status of cases was significantly higher as compared to controls, both mean levels were still far below sufficiency levels and the corresponding PTH levels were not significantly different from one another, and as such the interpretation is comparable. Furthermore we recently observed that PTH levels do not correlate with 25(OH) vitamin D levels in Saudis despite severely low levels .
The association of PTH with lipid concentrations, specifically the cholesterol levels was demonstrated earlier and we suggest that this is mainly due to the positive association of PTH to body composition and adiposity, rather than the cholesterol itself [19, 20]. Also, the inverse association of FT3 to circulating levels of 25(OH)D should be interpreted with caution. It was previously reported that higher vitamin D status was associated with low TSH only among younger individuals and not in adults . Furthermore, while vitamin D deficiency has been implicated in several autoimmune thyroid diseases, no association was elicited between the antibodies measured in the present study and vitamin D, confirming a recent study among Dutch natives about the lack of correlation of low vitamin D levels and the early stages of thyroid auto-immunity . Similarly, polymorphisms of vitamin D alpha-hydroxylase (CYP)1alpha, a key enzyme for regulating both systemic and tissue levels of 1, 25-dihydroxyvitamin D3 , did not correlate with known auto-immune disorders such as type 1 diabetes mellitus, Grave’s disease and Hashimoto’s thyroidits among Caucasian pedigrees . In this context, it should be taken into account that TSH may have direct and independent effects on bone metabolism regardless of thyroid hormones [25, 26].
The authors acknowledge several limitations. The cross-sectional nature of the study and the small sample size limits the findings of the study to at best, suggestive. Several major confounders were also not included including season which has a counterintuitive effect in the vitamin D status of citizens residing in the Gulf region . Gender difference could not be elicited due to the big discrepancy in the numbers between male and female subjects. Nevertheless, the study has its own strength as being the first to document several metabolic differences among Saudi patients with and without hypothyroid dysfunction and the first to associate vitamin D status in relation to thyroid function profile.
In summary, adult Saudi patients with subclinical hypothyroid dysfunction harbor several cardiometabolic abnormalities, including obesity and dyslipidemia, the latter being associated positively with PTH levels. Higher vitamin D levels were associated with lower FT3 in the patents. Further prospective studies are needed to determine whether the associations elicited are causal to one another.
The author would like to thank the nursing staff of KFMC for the recruitment, data and sample collection of patients. The authors also thank Mr. Benjamin Vinodson for the statistical analysis of the data.
- Akbar DH, Ahmed MM, Al-Mughales J: Thyroid dysfunction and thyroid autoimmunity in Saudi type 2 diabetics. Acta Diabetol. 2006, 43: 14-18. 10.1007/s00592-006-0204-8.View ArticlePubMedGoogle Scholar
- Abdullah MA, Salman H, Al-Habib S, Ghareeb A, Abanamy A: Antithyroid antibodies and thyroid dysfunction in Saudi children with Down syndrome. Ann Saudi Med. 1994, 14: 283-285.PubMedGoogle Scholar
- Bahammam SA, Sharif MM, Jammah AA, Bahammam AS: Prevalence of thyroid disease in patients with obstructive sleep apnea. Respir Med. 2011, 105: 1755-1760. 10.1016/j.rmed.2011.07.007.View ArticlePubMedGoogle Scholar
- Yasuda T, Okamoto Y, Hamada N, Miyashita K, Takahara M, Sakamoto F, Miyatsuka T, Kitamura T, Katakami N, Kawamori D, Otuski M, Matsuoka TA, Kaneto H, Shinomura I: Serum vitamin D levels are decreased and associated with thyroid volume in female patients with newly onset Grave’s disease. Endocrine. 2012, 42: 739-741. 10.1007/s12020-012-9679-y.View ArticlePubMedPubMed CentralGoogle Scholar
- Yasuda T, Okamoto Y, Hamada N, Miyashita K, Takahara M, Sakamoto F, Miyatsuka T, Kitamura T, Katakami N, Kawamori D, Otsuki M, Matsuoka TA, Kaneto TH, Shimomura I: Serum vitamin D levels are decreased in patients without remission of Grave’s disease. Endocrine. 2013, 43: 230-232. 10.1007/s12020-012-9789-6.View ArticlePubMedGoogle Scholar
- Rotondi M, Chiovato L: Vitamin D deficiency in patients with Grave’s disease: probably something more than a casual association. Endocrine. 2013, 43: 3-5. 10.1007/s12020-012-9776-y.View ArticlePubMedGoogle Scholar
- Rizzoli R, Boonen S, Brandi ML, Bruyere O, Cooper C, Kanis JA, Kaufman JM, Ringe JD, Weryha G, Reginster JY: Vitamin D supplementation in elderly or postmenopausal women: a 2013 update of the 2008 recommendations from the European Society for Clinical and Economic Aspects of Osteoporosis and Osteoarthritis (ESCEO). Curr Med Res Opin. 2013, 29: 305-313. 10.1185/03007995.2013.766162.View ArticlePubMedGoogle Scholar
- Amin A, Dhillo WS, Murphy KG: The central effects of thyroid hormones on appetite. J Thyroid Res. 2011, 2011: 306510-View ArticlePubMedPubMed CentralGoogle Scholar
- Al-Adsani H, Hoffer LJ, Silva JE: Resting energy expenditure is sensitive to small dose changes in patients on chronic thyroid hormone replacement. J Clin Endocrinol Metab. 1997, 82: 1118-1125. 10.1210/jc.82.4.1118.PubMedGoogle Scholar
- Muscogiuri G, Sorice GP, Mezza T: High normal TSH values in obesity: is it insulin resistance or adipose tissue’s guilt?. Obesity (Silver Spring). 2013, 21: 101-106. 10.1002/oby.20240.View ArticleGoogle Scholar
- Wanjia X, Chenggang W, Aihong W, Xiaomei Y, Jiajun Z, Chunxiao Y, Jin X, Yinglong H, Ling G: A high normal TSH levels is associated with an atherogenic lipid profile in euthyroid non-smokers with newly diagnosed asymptomatic coronary heart disease. Lipids Health Dis. 2012, 11: 44-10.1186/1476-511X-11-44.View ArticlePubMedPubMed CentralGoogle Scholar
- Williams GR: Extrathyroidal expression of TSH receptor. Ann Endocrinol (Paris). 2011, 72: 68-73. 10.1016/j.ando.2011.03.006.View ArticleGoogle Scholar
- Wang F, Tan Y, Wang C, Zhang X, Zhao Y, Song X, Zhang B, Guan Q, Xu J, Zhang J, Zhang D, Lin H, Yu C, Zhao J: Thyroid-stimulating hormone levels within the reference range are associated with serum lipid profiles independent of thyroid hormones. J Clin Endocrinol Metab. 2012, 97: 2724-2731. 10.1210/jc.2012-1133.View ArticlePubMedGoogle Scholar
- Palmieri EA, Fazio S, Lombardi G, Biondi B: Subclinical hypothyroidism and cardiovascular risk: a reason to treat?. Treat Endocrinol. 2004, 3: 233-244. 10.2165/00024677-200403040-00005.View ArticlePubMedGoogle Scholar
- Al-Daghri NM, Al-Attas OS, Al-Okail MS, Alkharfy KM, Al-Yousef MA, Nadhrah HM, Sabico SB, Chrousos GP: Severe hypovitaminosis D is widespread in Saudi adults and is more common in non-diabetics than diabetics. Saudi Med J. 2010, 31: 775-780.PubMedGoogle Scholar
- Al-Daghri NM, Al-Attas OS, Alokail MS, Alkharfy KMK, Yousef M, Nadhrah HM, Al-Othman A, Al-Saleh Y, Sabico S, Chrousos GP: Hypovitaminosis D and cardiometabolic risk factors among non-obese youth. Cent Eur J Med. 2010, 5: 752-757. 10.2478/s11536-010-0045-2.Google Scholar
- Tamer G, Mesci B, Tamer I, Kilic D, Arik S: Is vitamin D deficiency an independent risk factor for obesity and abdominal obesity in women?. Endokrynol Pol. 2012, 63: 196-201.PubMedGoogle Scholar
- Al-Saleh Y, Al-Daghri NM, Alkharfy KM, Al-Attas OS, Alokail MS, Al-Othman A, Sabico S, Chrousos GP: Normal circulating PTH in Saudi healthy individuals with hypovitaminosis D. Horm Metab Res. 2013, 45: 43-46.PubMedGoogle Scholar
- Williams DM, Fraser A, Lawlor DA: Association of vitamin D, parathyroid hormone and calcium with cardiovascular risk factors in US adolescents. Heart. 2011, 97: 315-320. 10.1136/hrt.2010.203224.View ArticlePubMedGoogle Scholar
- Kayaniyil S, Vieth R, Harris SB, Retnakaran R, Knight JA, Gerstein HC, Perkins BA, Zinman B, Hanley AJ: Association of 25(OH)D and PTH with metabolic syndrome and its traditional and nontraditional components. J Clin Endocrinol Metab. 2011, 96: 168-175. 10.1210/jc.2010-1439.View ArticlePubMedGoogle Scholar
- Chailurkit LO, Aekplakorn W, Ongphiphadhanakul B: High vitamin D status in younger individuals is associated with low circulating thyrotropin. Thyroid. 2013, 23: 25-30. 10.1089/thy.2012.0001.View ArticlePubMedGoogle Scholar
- Effraimidis G, Badenhoop K, Tijssen JG, Wiersinga WM: Vitamin D deficiency is not associated with early stages of thyroid autoimmunity. Eur J Endocrinol. 2012, 167: 43-48. 10.1530/EJE-12-0048.View ArticlePubMedGoogle Scholar
- Kozai M, Yamamoto H, Ishiguro M, Harada N, Masuda M, Kagawa T, Takei Y, Otani A, Nakahashi O, Ikeda S, Taketani Y, Takeyama K, Kato S, Takeda E: Thyroid hormones decrease plasma 1α,25-dihydroxyvitamin D levels through transcriptional repression of the renal 25-hydroxyvitamin D3 1α-hydroxylase gene (CYP27B1). Endocrinology. 2013, 154: 609-622. 10.1210/en.2012-1647.View ArticlePubMedGoogle Scholar
- Pani MA, Regulla K, Segni M, Krause M, Hofmann S, Hufner M, Herwig J, Pasquino AM, Usadel KH, Badenhoop K: Vitamin D1alpha-hydroxylase (CYP1alpha) polymorphism in Grave’s disease, Hashimoto’s thyroidits and type 1 diabetes mellitus. Eur J Endocrinol. 2002, 146: 777-781. 10.1530/eje.0.1460777.View ArticlePubMedGoogle Scholar
- Mazziotti G, Porcelli T, Patelli I, Vescovi PP, Giustina A: Serum TSH values and risk of vertebral fractures in euthyroid post-menopausal women with low bone mineral density. Bone. 2010, 46: 747-751. 10.1016/j.bone.2009.10.031.View ArticlePubMedGoogle Scholar
- Maziotti G, Sorvillo F, Piscopo M, Cioffi M, Pilla P, Biondi B, Iorio S, Giustina A, Amato G, Carella C: Recombinant human TSH modulates in vivo C-telopeptides of type-1 collagen and bone alakaline phosphatase, but not osteoprotegerin production in postmenopausal women monitored for differentiated thyroid carcinoma. J Bone Miner Res. 2005, 20: 480-486.View ArticleGoogle Scholar
- Al-Daghri NM, Al-Attas OS, Alokail MS, Alkharfy KM, El-Kholie E, Yousef M, Al-Othman A, Al-Saleh Y, Sabico S, Kumar S, Chrousos GP: Increased vitamin D supplementation recommended during summer season in the gulf region: a counterintuitive seasonal effect in vitamin D levels in adult, overweight and obese Middle Eastern residents. Clin Endcrinol (Oxf). 2012, 76: 346-350. 10.1111/j.1365-2265.2011.04219.x.View ArticleGoogle Scholar
- The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6823/13/31/prepub
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