Fig. 4

Effects of Act on renal tissue and podocytes. (a-d) The Bcl-2, Bax, and cleaved caspase-3 expression in mice kidney tissue; (e) TUNEL assay in mice renal tissues (Scale bar = 50 μm); DAPI, blue, apoptotic cell, red. (f) Real-time RT-PCR quantitation of WT1、Bax、bcl-2. (g) Differences in apoptosis of MPC5 cells between normal glucose, high glucose and Act interventions: Q1 is necrotic cells, Q2 is late apoptotic cells, Q3 is early apoptotic cells and Q4 is the number of viable cells. The data were exhibited as means ± SD. #P < 0.05, ##P < 0.01, and ###P < 0.001 vs. db/m group; *P < 0.05, **P < 0.01, and ***P < 0.001 vs. db/db group