Fig. 4

UBE3A knockdown increased the phosphorylation of AMPK through decreasing the ubiquitination of AMPK in the KGN cells

(A) The protein levels of p-AMPK and AMPK were detected by western blot after UBE3A knockdown. (B) The protein stability of AMPK detected by western blot after UBE3A knockdown. (C) Exogenous interaction between AMPK and UBE3A was determined using co-IP with anti-Flag or anti-HA antibodies co-transfected with Flag-UBE3A and HA-AMPK. (D) Endogenous interaction between AMPK and UBE3A was determined using co-IP with anti-AMPK or anti-UBE3A antibodies. (E) The ubiquitination of AMPK after UBE3A knockdown was detected by western blot