Fig. 6

The apoptosis of HUVECs from GDM-D and GDM-I. Western blot analysis of cleaved caspase-3 (A, n = 5 per group), cleaved PARP (B, n = 9 per group) expression and representative membrane blots (C). H-scores of cleaved caspase-3 and representative photographs of immunohistochemistry staining in the indicated groups (E and F). Annexin V/PI assay of HUVECs from Normal (n = 3), GDM-D (n = 3) and GDM-I (n = 3) (E and G). Histograms showing the apoptosis rates in the indicated groups. The results were analyzed with one-way ANOVA test. (∗P < 0.05, ∗∗P < 0.01 compared with normal; #P < 0.05, ##P < 0.01 compared with GDM-D).