α-NETA down-regulates CMKLR1 mRNA expression in ileum and prevents body weight gains collaborating with ERK inhibitor PD98059 in turn to alleviate hepatic steatosis in HFD-induced obese mice but no impact on ileal mucosal integrity and steatohepatitis progression

Table 3 CMKLR1, ZO-1 and pERK expressions in ileal tissues of group ND, group NETA, group PD, group NETA + PD, and group HFD

Gene	Group	mRNA level^a	Protein level^b
CMKLR1	Group ND	1.00 ± 0.62	No detectable
	Group NETA	0.75 ± 0.61^d	No detectable
	Group PD	1.76 ± 2.12	No detectable
	Group NETA + PD	5.18 ± 8.03	No detectable
	Group HFD	4.17 ± 1.84^c	No detectable
ZO-1	Group ND		0.99 ± 0.24
	Group NETA		0.67 ± 0.52
	Group PD		1.21 ± 1.38
	Group NETA + PD		0.55 ± 0.47
	Group HFD		1.63 ± 1.50
pERK	Group ND		0.79 ± 0.39
	Group NETA		0.75 ± 0.38
	Group PD		1.78 ± 0.88
	Group NETA + PD		0.31 ± 0.08
	Group HFD		0.83 ± 0.34

^amRNA abundance was analyzed using the 2-^ΔΔct Ct method with GAPDH as the constitutive marker
^bThe protein expression was analyzed using integrated intensity with β-actin as the constitutive marker
^cP < 0.05 compared with group ND
^dP < 0.01 compared with group HFD

ISSN: 1472-6823