Table 2 Primer sequences used for quantitative real-time PCR
Gene symbol | Forward sequence 5'-3' | Reverse sequence 5'-3' | Annealing temperature (°C) |
|---|---|---|---|
Kisspeptin | TGCTGCTTCTCCTCTGTGTCGC | CAGGCTTGCTCTCTGCATACCGC | 58 |
GAPDH (Ref) | GAACATCATCCCTGCATCCA | CCAGTGAGCTTCCCGTTCA | 58 |