Table 2 Forward and Reverse Primers Used for Real-Time Quantitative PCR
Gene | Primer | Product size (bp) | Annealing temperature (C) |
|---|---|---|---|
IL-6 | F: GGTACATCCTCGACGGCATCT R: GT GCCTCTTTGCTGCTTTCAC | 250 | 60 |
IL-10 | F: AAGGCAGTGGAGCAGGTGAA R: CCAGCAGACTCAATACACAC | 250 | 58 |
CD27 | F: TGTCGGCACTGTAACTCTGGTCT R: CCTGCACTGCCAGCCAT | 250 | 59 |
CXCL13 | F: GCTTGAGGTGTAGATGTGTCC R: CCCACGGGGCAAGATTTGAA | 150 | 59 |
IFN-γ | F: GGCATTTTGAAGAATTGGAAAG R: TTTGGATGCTCTGGTCATCTT | 286 | 60 |
TGF-β1 | F: CCCAGCATCTGCAAAGCTC R: GTCAATGTACAGCTGCCGCA | 286 | 60 |