Fig. 2
From: Metformin activates Wnt/β-catenin for the treatment of diabetic osteoporosis
Metformin enhances the osteogenic differentiation ability of MC3T3-E1 cells under HG conditions. A ARS was applied for the assessment of calcification in MC3T3-E1 cells in each group. B ALP colorimetric assay kit was utilized to measure the activity of ALP in each group of cells; C/D Western blot was performed for the detection of the expression of osteogenic differentiation-related genes (Runx2, OCN) in each group of cells, **P < 0.01, vs. Control; ##P < 0.01, vs. HG