Fig. 6

a Effect of metformin on AMPK phosphorylation in mock, reference OCT-1 and mutant transfects: Representative ummunoblots showing the change in pAMPK expression by the presence/absence of metformin in HepG2 cells expressing vehicle control, reference OCT-1 and mutant OCT-1 constructs. The lower panel represents the effect of the same on the expression of β–actin protein used as a loading control. M1: 181C > T; M2; 1201G > A; M3: 1222A > G. b Graph showing normalised densitometric values for the metformin- treated and untreated mock, wild type cells and mutant cells