Fig. 6

MAPK1 is a functionally downstream effector of miR-761. A Effects of the transfection of miR-761 mimic+MAPK1 expressing construct, miR-761 mimic+pcDNA control, miR-761 mimic and miR-NC mock on MAPK1 protein expression in SW579 and TPC-1 cells, with GAPDH as a loading control for expression quantification. B MTS assay of the viability of SW579 and TPC-1 cells after the indicated transfections. C Proliferation rate of SW579 and TPC-1 cells after the indicated transfections, based on the staining with EdU and DAPI. D Apoptosis rate of SW579 and TPC-1 cells after the indicated transfections, based on the Annexin V/PI staining. E Effects of the indicated transfections on the invasion ability of SW579 and TPC-1 cells determined by transwell assay. F Effects of the indicated transfections on the migration rate of SW579 and TPC-1 cells examined by wound-healing assay. G and H Immunoblotting of the protein levels of Cleaved-caspase-3 and MMP9 in SW579 and TPC-1 cells after the indicated transfections, with GAPDH as a loading control for relative protein quantification. **P < 0.01, ***P < 0.001, ****P < 0.0001