Fig. 2

MIR31HG depletion affects cell proliferation, apoptosis, invasion and migration in vitro. A Effect of si-MIR31HG or si-NC introduction on MIR31HG expression in SW579 and TPC-1 cells gauged by qRT-PCR, normalized to β-actin expression. B Effect of si-MIR31HG or si-NC transfection on cell viability determined by MTS assay. C Effect of si-MIR31HG or si-NC transfection on the proliferation rate of SW579 and TPC-1 cells, based on the staining with EdU and DAPI. Scale bars, 50 μm. D Representative images depicting a cell apoptosis assay and apoptosis rate of si-MIR31HG-transfected or si-NC-introduced SW579 and TPC-1 cells by flow cytometry, based on the Annexin V/PI staining. E Effect of si-MIR31HG or si-NC transfection on the invasion rate of SW579 and TPC-1 cells as measured by transwell assay. F Representative pictures showing the migration ability of si-MIR31HG-transfected or si-NC-introduced SW579 and TPC-1 cells as evaluated by wound-healing assay. G and H The protein levels of Cleaved-caspase-3 and MMP9 in si-MIR31HG-transfected or si-NC-introduced SW579 and TPC-1 cells by immunoblotting. GAPDH served as a loading control for relative protein quantification. **P < 0.01, ***P < 0.001, ****P < 0.0001