Fig. 5

MAPK14 siRNA was transfected into GH3 cells to detect the effect of MAPK14 protein on the expression of PRL, NF-κB p65, Bcl2 and Bax in GH3 cells regulated by bromocriptine. a Western blotting was used to analyse the knockdown efficiency of three MAPK14 siRNA (100 nM) transfected GH3 cells for 72 h. b The level of PRL in the supernatant of GH3 cells was detected by ELISA.^**p < 0.01, ^****p < 0.0001 (n = 6). c, d The protein expression levels of PRL, NF-κB p65, Bcl2 and Bax in GH3 cells were analysed by western blotting.^*p < 0.05, ^**p < 0.01, ^***p < 0.001,^****p < 0.0001 (n = 3)