Skip to main content
Fig. 7 | BMC Endocrine Disorders

Fig. 7

From: LncRNA HCP5 knockdown inhibits high glucose-induced excessive proliferation, fibrosis and inflammation of human glomerular mesangial cells by regulating the miR-93-5p/HMGA2 axis

Fig. 7

HMGA2 overexpression abolished the effects of mIR-93-5p restoration. A The availability of HMGA2 overexpression vector was checked using western blot. In the miR-NC, miR-93-5p, miR-93-5p + vector and miR-93-5p + HMGA2 groups, (B) cell proliferation was monitored using CCK-8 assay. C and D Cell apoptosis was investigated using flow cytometry assay. E The levels of Cleaved-PARP and Cleaved-caspase 3 were quantified using western blot. F The protein levels of FN, Col IV and Col I were checked by western blot. G The release of TNF-α, IL-6 and IL-1β was ascertained using ELISA kits. *P < 0.05, **P < 0.01 and ***P < 0.001

Back to article page

BMC Endocrine Disorders

ISSN: 1472-6823

Contact us