Fig. 3

Overexpressed NEU1 reversed the effects of miR-23b-3p upregulation on Treg and Th17 cell percentage. a-d Percentages of Treg cells (CD4⁺ CD25⁺ Foxp3⁺) and Th17 cells (CD4⁺ IL-17⁺) after obesity-induced IR model construction and injection of lentivirus carriers for miR-23b-3p mimic and NEU1 overexpression plasmid were calculated by flow cytometry. e-f Protein/GAPDH expressions of TGF-β1, IL-10, IL-17 and IL-6 after obesity-induced IR model construction and injection of lentivirus carriers for miR-23b-3p mimic and NEU1 overexpression plasmid were measured with Western blot. GAPDH was employed as internal control. All experiments have been performed in triplicate and data were expressed as mean ± standard deviation (SD). ^***P < 0.001, vs. Control; ^{^^^}P < 0.001, vs. MC; ^##P < 0.01, ^###P < 0.001, vs. negative control (NC); ^ΔΔP < 0.01, ^ΔΔΔP < 0.001, vs. mimic (M); ^ξξξP < 0.001, vs. NEU1. Treg cells: regulatory T cells; Th17 cells: T helper 17 cells; TGF-β1: Transforming growth factor-β1