Table 3 Primers used for qRT-PCR assessment of gene expressions
Gene | Primers | Primer sequence | Tm |
|---|---|---|---|
Cox-1 | Forward | 5-GGTAACTGCTTAGGACCAGT-3 | 58 |
Reverse | 5-ACTGTTCTCCGTACCTTCAC-3 | ||
Cox-2 | Forward | 5-TGTCATTCCAGAGTGCTGAG-3 | 58 |
Reverse | 5-GGCTAACAAGGAGTTCAGCA-3 | ||
Beta-Actin | Forward | 5-GAT CTC CTT CTG CAT CCT GT-3′ | 57 |
Reverse | 5′-TGG GCA TCC ACG AAA CTA C- 3’ |