Fig. 4

MAPK14 siRNA reduces PRL production in GH3 cells. a Effect of MAPK14 siRNA (30, 50 or 100 nM) on MAPK14 mRNA expression levels in GH3 cells. ^*P < 0.05 and ^**P < 0.01 vs. NC, (n = 3). b Effect of MAPK14 siRNA (30, 50 or 100 nM) on PRL mRNA expression levels in GH3 cells. ^**P < 0.01 and ^***P < 0.001 vs. NC, (n = 3). c Effect of MAPK14 siRNA (30, 50 or 100 nM) on MAPK14 and PRL protein expression levels in GH3 cells. d and e Relative protein expression of both MAPK14 and PRL were quantified and normalized to β-actin. ^**P < 0.01 vs. NC, (n = 3)