CRISPR-Cas9-mediated knockout of SPRY2 in human hepatocytes leads to increased glucose uptake and lipid droplet accumulation

Table 1 Top 20 DE genes in RNA-Seq analysis of HepG2 mock and SPRY2 KO cells

Symbol	Gene Name	HepG2
Symbol	Gene Name	log2FC	Adjusted P
PLA2G2A	Phospholipase A2 group IIA	3.86	1.43E-20
GADD45B	Growth arrest and DNA damage inducible beta	0.23	2.00E-20
CYP1A1	Cytochrome P450 family 1 subfamily A member 1	0.26	2.28E-16
SPARC	Secreted protein acidic and cysteine rich	0.29	5.24E-16
MT1X	Metallothionein 1X	0.30	2.11E-15
RBM23	RNA binding motif protein 23	0.16	3.43E-15
SNORA53	Small nucleolar RNA, H/ACA box 53	5.05	1.92E-12
AKR1B1	Aldo-Keto reductase family 1 member B	0.37	2.19E-12
TNFAIP3	TNF alpha induced protein 3	0.34	2.19E-12
IFI6	Interferon alpha inducible protein 6	2.94	5.43E-12
AKR1B10	Aldo-Keto reductase family 1 member B10	0.38	6.03E-12
ZSCAN4	Zinc finger and SCAN domain containing 4	0.25	3.70E-11
NFKBIA	NFKB inhibitor alpha	0.39	9.43E-11
MT1E	Metallothionein 1E	0.39	1.22E-10
DPP4	Dipeptidyl peptidase 4	0.36	1.66E-10
TRIM31	Tripartite motif containing 31	0.05	2.17E-10
PEG10	Paternally expressed 10	2.41	1.07E-09
TNF	Tumour necrosis factor	0.11	5.71E-09
TSPAN7	Tetraspanin 7	0.31	5.75E-09
FGL1	Fibrinogen like 1	2.48	6.23E-09

Values are based on the lowest adjusted P values and log2 fold changes (log2FC) ≥ 2.0

ISSN: 1472-6823