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Fig. 5 | BMC Endocrine Disorders

Fig. 5

From: CRISPR-Cas9-mediated knockout of SPRY2 in human hepatocytes leads to increased glucose uptake and lipid droplet accumulation

Fig. 5

Validation of RNA-Seq results by RT-qPCR. Relative mRNA level in HepG2 mock, GFP and SPRY2 KO cells as assessed by RT-qPCR, for: a PLA2G2A, b DPP4, c GADD45B, d TNFAIP3, e CYP1A1 and f AKR1B1. Data were analysed in qbase+ and normalised to HPRT and RPL13, determined by geNorm to be the most stable reference genes. Bars show mean + SD from 3 biological replicates each, quantified in triplicate and expressed relative to mock. *P < 0.05, **P < 0.01, ***P < 0.001 by one-way ANOVA with Dunnett’s post-hoc test to compare all bars to mock

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BMC Endocrine Disorders

ISSN: 1472-6823

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