Fig. 1

Confirmation of CRISPR-Cas9 genome editing for SPRY2. Near-infrared Western blotting was carried out to simultaneously detect SPRY2 and β-tubulin in HepG2 untreated (mock) and CRISPR-Cas9 genome edited (KO/OE) cell lysates. SPRY2 band density was quantified in Fiji, normalised to β-tubulin and expressed as a percentage of mock. a Representative Western blot for KO study and b band density analysis showing mean + SD of 2 independent transductions. ‘GFP’ denotes transduction control cells expressing GFP using the pLJM1-EGFP lentiviral vector. c Western blot for SPRY2 overexpression (OE) study and d corresponding band density analysis. Full-length blots are shown in Additional file 1: Figure S8