Fig. 5

Diabetes-induced changes in α-SMA and pHH3 expression in the kidney of Wt and Hif1α ^+/− mice. a Confocal imaging of 8 μm sections of the kidney stained with anti-α-SMA and anti-pHH3 antibodies. Images are stacked Z-plane sections; nuclei are counterstained with Hoechst 33,342 (blue); scale bars 50 μm. b Quantification of α-SMA⁺ area was done per glomerular area (10 glomerulus/each section) and the positive pHH3⁺ staining was determined as a number of the positive nuclei in the field of view of the renal cortex. The values represent means ± SEM (n = 3 sections/3mice/group). Statistical significance assessed by two-way ANOVA: diabetes effect in the expression of pHH3 and α-SMA (P < 0.0001). Post hoc pairwise comparison **P < 0.01, ***P < 0.001. Abbreviations: alpha smooth muscle actin (α-SMA), phospho-histone H3 (pHH3)