Fig. 3

Gene expression changes in the renal cortex of diabetic and non-diabetic Wt and Hif1α ^+/− mice. a The relative gene expression changes were analyzed using RT-qPCR and quantified with ΔΔCT method. The values represent means ± SEM (n = 8 samples/group). Statistical significance differences in normalized Ct values were tested by two-way ANOVA followed by post hoc pairwise comparison tests *P < 0.05, **P < 0.01, ***P < 0.001. b The renal cortex extracts were prepared and probed on Western blots with antibodies directed against connexin 43 (CX43; ~43 kDa). Representative immunoblot for CX43 is shown. c Combined results obtained by densitometric evaluation of the Western blots of CX43 using cortical extracts from three mice per group. Ponceau S staining was used as the loading control. Data were analyzed by ImageJ software. Statistical significance differences were tested by two-way ANOVA followed by post hoc pairwise comparison tests *P < 0.05, **P < 0.01. Abbreviations: adrenomedullin (Adm), podocin (Nphs2), SRY (Sex Determining Region Y)-Box 9 (Sox9)), transforming growth factor beta 1 (Tgfβ1), fibronectin 1 (Fn1), connective tissue growth factor (Ctgf), netrin (Ntn1), connexin 43 (Cx43), and pyruvatdehydrogenase kinase1 (Pdk1)