Fig. 2

PAS and trichrome-staining of the renal cortex in non-diabetic and diabetic Wt and Hif1α ^+/− mice. a Representative PAS staining of 8 μm kidney sections showed advanced glycation end products in diabetic Wt (Wt STZ) and diabetic Hif1α ^+/− (Hif1α ^+/− STZ). The strongest positive staining was detected in the tubular part of the kidney section. b Delineated PAS⁺ area in the kidney section using Adobe Photoshop. c Representative Masson’s trichrome-staining of 8 μm kidney sections showed increased fibrosis with increased collagen fibers in the renal cortex of diabetic kidneys. d Delineated collagen⁺ area in the kidney section by ImageJ. e-f A relative quantification of staining was determined as a percentage of positive area in the field of view by ImageJ. Scale bar 100 μm. The values represent means ± SEM (n = 3 sections/3 samples/group). Statistical significance differences were tested by two-way ANOVA (diabetes effect: PAS and trichrome (P < 0.0001); effect of genotype in PAS staining (P = 0.04)). *Significant differences by post hoc pairwise comparison, *P < 0.05, **P < 0.01, ***P < 0.0001