Fig. 1

Role of MuRF3 in regulating PPAR isoform activity and its role in high fat diet cardiac hypertrophy in vivo. Isolation of cardiac nuclei from MuRF3−/− and sibling wildtype mouse hearts revealed increases in a. PPARα, PPARβ/δ, and PPARγ activity using PPRE-DNA as bait and ELISA detection of PPARα protein (N = 3-4/group as indicated in bars). b. Experimental design of high fat diet (60 %)-induced cardiomyopathy. c. High fat diet induces cardiac MuRF3 levels after 26 weeks HFD (N = 3/group). d. MuRF3−/− heart weights 26 weeks high fat diet normalized to body weight and tibia length (N = 3/group). e. MuRF3−/− body weight at baseline and after high fat diet for 26 weeks (N indicated below graph). Values expressed as Mean ± SE. Statistical analysis was performed using a Student’s t-test comparing MuRF3−/− and MuRF3+/+ groups. *p ≤ 0.001, **p < 0.01. #p < 0.05