Figure 1
AIF-1 secretion from WAT in vitro . Pieces of WAT (300 mg) were incubated in Krebs-Ringer phosphate buffer (pH 7.4) supplemented with 40 g/liter of defatted bovine serum albumin and 1 g/liter of glucose (3 mL) and the concentration of AIF-1 in medium was determined by ELISA, see “Protein measurements” section for details (cohort 1; n = 5 women, 44 ± 3 years, BMI 33 ± 11 kg/m2). One sample at the 3 h incubation time point was lost during sample processing.