Transcripts | Primer | Primer Sequences (5’-3’) | Thermocycling Condition | PCR Mix |
---|---|---|---|---|
GAPDH | Forward | AGTGCCAGCCTC GTCTCATA | 95 °C/10 min, 40 cycles at 95 °C/15s, 58 °C/20s, and 72 °C/30 s | SYBR green Master Mix (10 µl; 2x concentration), Forward primers: 0.8 µl and 10 pmole, Reverse primers: 0.8 µl and 10 pmole, Nuclease-free DEPS Water: 6.4 µl, Template cDNA: 2 µl |
Reverse | GAGAAGGCAGCCCTGGTAAC | |||
TGF-β | Forward | TGACATGAACCGACCCTTCC | 95 °C/10 min, 40 cycles at 95 °C/15s, 60 °C/20s, and 72 °C/30 s | |
Reverse | TGCCGTACACAGCAGTTCTT | |||
Apelin | Forward | CTCTGGCTCTCCTTGACTGC | 95 °C/10 min, 40 cycles at 95 °C/15 s, 59 °C/20s, and 72 °C/30s | |
Reverse | TCGAAGTTCTGGGCTTCACC |