Fig. 5

Circ_072697 regulated KDM2A expression by sponging miR-3150a-3p. A: RIP assay was employed to assess the interaction of KDM2A and miR-3150a-3p. B: The luciferase activities in HaCaT cells co-transfected with miR-3150a-3p NC or miR-3150a-3p mimics. C: qRT-PCR of KDM2A expression in normal and DFU tissues. D: Western blot analysis of KDM2A expression in normal and DFU tissues. E: qRT-PCR analysis of KDM2A expression in AGE-treated HaCaT cells transfected with si-NC + inh-NC, si-circ_072697#1 + inh-NC, si-NC + miR-3150a-3p inh, or si-circ_072697#1 + miR-3150a-3p inh. ** p < 0.01, *** p < 0.001