Table 2 Sequences of the primers

BRAF at Chr7q34: 140,433,812-140,624,564(190,752 bp)

exon15: 176,372-176,490 (119 bp) K601E: 176,431(A → G) rs121913364: 140,453,134 V600E: 176,429 (T → A) rs113488022: 140,453,136

5′-TGCTTGCTCTGATAGGAAAATG-3′ 5′-CCACAAAATGGATCCAGACA-3′

Chr7:140,453,250-140,453,078 (173 bp) intron: 176,315-176,371 exon15:176,372-176,487 (116 bp)

RET at Chr10q11.2: 43,572,517-43,625,799

exon5: 34,308-34,503 (196 bp) R313Q: 34,378(G → A) rs77702891: 43,601,894 R330Q: 34,429(G → A) rs80236571: 43,601,945

5′-CTTTCCTCACAACCCCCTCC-3′ 5′-AGAGCGAGCACCTCATTTCC-3′

Chr10: 43,601,341-43,602, 077 (737 bp) intron: 33, 825–34,307&34,504-34,561 exon5: 34,308-34,503 (196 bp) STS: 33,825-34,398

exon8: 40,031-40,156 (126 bp) G533C: 40,105(G → T) rs75873440: 43,607,621

5′-CCTGTGCAGTCAGCAAGAGA-3′ 5′-CCTGTTCCCATGCCCTGATT-3′

Chr10: 43,607,577-43,608,444 (868 bp) exon8: 40,061-40,155 (96 bp) intron: 40,156-40,784&40,896-40,928 exon9: 40,785-40,895 (111 bp)

exon10: 41,488-41,607(120 bp) C609R: 41,553(T → C) rs77558292: 43,609,069 C609Y: 41,554(G → A) rs77939446: 43,609,070 C611W: 41,561 (C → G) rs80069458: 43,609,077 C618R/G: 41,580(T → C/G) rs76262710: 43,609,096 C618S: 41,581(G → C) rs79781594: 43,609,097 C620R: 41,586(T → C) rs77316810: 43,609,102 C620F/S/Y: 41,587(G → A/C/T) rs77503355: 43,609,103 C620W: 41,588(C → G) rs79890926: 43,609,104 cds-indel: 41,562_41,588del27 rs121913313: 43,609,078_43,609,104del27

5′-GGAAACCTGGATCCCACAGG-3′ 5′-GGGAGGGAAGTTTCATGGGG-3′

Chr10: 43,608,459-43,609,249 (791 bp) intron: 40,943-41,487&41,608-41,557 exon10: 41,488-41,607(120 bp) STS: 41558-41733

exon12: 44,516-44,663 (148 bp)

5′-GTGGGCCCAATGTGTGGATA -3′ 5′-CTCTTCAGGGTCCCATGCTG-3′

Chr10: 43,611,512-43,612,272 (761 bp) intron: 43,996-44,515&44,664-44,756 exon10: 44,516-44,663 (148 bp)

exon13: 46, 305–46,412 (108 bp) S765P: 46,313(T → C) rs75075748: 43,613,829 E768E: 46,324(G → A/C) rs78014899: 43,613,840 V778I: 46,352(G → A) rs75686697: 43,613,868 L790F: 46,390(G → C) rs75030001: 43,613,906 Y791F: 46,392(A → T) rs77724903: 43,613,908

5′-CGGGGAATTTCTGTGGACGA-3′ 5′-ATGGCAGTGTCACACCAGAG-3′

Chr10: 43,613,496-43,614,200 (705 bp) intron: 45,980-46,304&46,413-46,684 exon13: 46, 305–46,412 (108 bp) misc_difference: 46,327

exon14: 47, 463–47,677 (215 bp) V804M: 47,480(G → A/T) rs79658334: 43,614, 996

5′-GAGGCAGAGAGCAAGTGGTT-3′ 5′-AATAGCACGAGTCGTCAGGC-3′

Chr10: 43,614,767-43,615,517 (751 bp) intron: 47,251-47, 462&47,678-48,001 exon14: 47, 463–47,677 (215 bp)

exon15: 48,013-48,135 (123 bp) S891A: 48,076(T → G) rs75234356: 43,615,592 cds-indel: 48,051_48,053delAGCinsTTT rs121913306 43,615,567_43,615,567delins R897Q: 48,095(G → A) rs76087194: 43,615,611 cds-indel: 48,097_48,108del12 rs121913309: 43,615,613_43,615,624del12

5′-TCTCACAGGGGATGCAGTATCTG-3′ 5′-GAGGCTGAGCGGAGTTCTAATTG-3′

Chr10: 43,615,159-43,615,837 (679 bp) exon14: 47,643-47,677 (35 bp) intron: 47,678-48,012&48,136-48,321 exon15: 48,013-48,135 (123 bp)

exon16: 49, 878–49,948 (71 bp) M918T: 49,900(T → C) rs74799832: 43,617,416 R912P: 49,882(G → C/T) rs78347871: 43,617,398

5′-GCTCCAGCCCCTTCAAAGAT-3′5′-CTTTGAGCAGTTTGGGGCAC-3′

Chr10: 43,617,229-43,617,941 (713 bp) intron: 49,713-49, 877&49, 949–50,425 exon16: 49,878-49,948 (71 bp) STS: 49,832-50,007

exon17: 51, 603–51,740 (138 bp)R972G: 51,715(A → G) rs76534745: 43,619,231

5′-CTCTGATGGGAGTGGCTTGG-3′5′-CCACTCAGGCACCCCTTAAC-3′

Chr10: 43,618,871-43,619,601 (713 bp) intron: 51,355-51, 602&51,741-52,085 exon17: 51, 603–51,740 (138 bp)

2q35

DIRC3 (noncoding RNA):218,148,746-218,621,316(472571 bp)rs966423:218,310,340

5′-CGGCCTCGACCAACACTTAT-3′ 5′-ACTGGGCGTCTCAACTACAATCTG -3′

Chr2: 218,310,115-218,310,537(423 bp) located in the intron region of DIRC3,

5q24

Pre-miR-146a: 159,912,359-159,912,457(99 bp) rs2910164: 159,912,418

5′-ATTTTACAGGGCTGGGACAG-3′ 5′-TCTTCCAAGCTCTTCAGCAG-3′

Chr5: 159,912,297-159,912,523(227 bp)

8q24

rs4733616: 128,662,095

5′-CACCGGGGATTGGAAGAGATAAG-3′ 5′- TGAAGCCACAGGGGAGAAAAGT -3′

Chr8:128,661,750-128,662,159(410 bp)

8p12

NRG1 transcript variant HRG-beta1c: 31,496,820-32,622,558(1,125,738 bp) rs2439302: 32,432,369

5′-AATGCAAGAATGGCCTAACACAAT-3′ 5′-AACCTGGGGSSSSSTCTGAAGC-3′

Chr8: 32,432,326-32,432,660(334 bp) located in intron of NRG1

9q22.33

rs965513:100,556,109

5′-CCGGCTTGAGTTCAGGTATGTAGT-3′ 5′-CCAGGCTCAGGTTATGTCTTTGTT-3′

Chr9: 100,555,758-100,556,177(420 bp)

9q22

FoxE1: 100,615,537-100,618,997(3,460 bp)rs1867277: 100,615,914

5′-AGACCAGCTGCAGCCACCCCAACC-3′ 5′-GTCTCGCCGCGCTCTTCCTTCACG-3′

Chr9: 100,615,806-100,616,270(465 bp)located in the STS of FoxE1

14q13.3

rs944289: 36,649,246

5′-CCAGTGGCCCCGCAGGTT-3′5′-GAAAAGCACGTCTCCCCACAGTCC-3′

Chr14: 36,648,944-36,649,435(492 bp)

rs116909374: 36,738,361

5′-TGTAATGGCAGCTCTTGACCTT-3′ 5′-ACCTTTGATTGCCCTTAGTTTGA-3′

Chr14: 36,738,229-36,738,674(446 bp)