Fig. 5

HFD increased lymphocyte infiltration in the thyroid. A Representative images of CD45 (mainly lymphocytic lineage), CD4 (CD4⁺ T cell), CD8 (CD8⁺ T cell) and CD19 (B cells) staining of thyroid sections. Each right panel (magnification ×2000) depicted a magnified image of the boxed area (red) of the corresponding image in the middle panel (magnification ×400). B Quantification of the data displayed in (A) revealed an increased infiltration of thyroid parenchyma by the CD45⁺ cells, CD4⁺ T cells, CD8⁺ T cells and B cells. Cells have been randomly counted in five nonoverlapping fields (magnification ×400) of tissue sections from 5 rats per group. Values were mean ± SEM; ^**p < 0.01