Table 1 The primers and PCR product size for 4 SNPs in the ADIPOQ gene
ID | Major/minor allele | Primer (sequence 5′ to 3′) | Restrection Enzyme | PCR Restrection | RFLP fragments (bp) |
|---|---|---|---|---|---|
rs17300539 | G/A | F: 5′-AGGCTCTGTGTGGACTGTGGA-3′ R: 5′-CCTGGAGAACTGGAAGCTGC-3′ | MspI | 296 | AA = 296 GA = 129,167,296 GG = 129,167 |
rs266729 | G/C | F: 5′-AGGCTCTGTGTGGACTGTGGA-3′ R: 5′-CCTGGAGAACTGGAAGCTGC-3’ | HhaI | 296 | CC = 296 GC = 113,183,296 GG = 113,183 |
rs1501299 | G/T | F: 5’- GGCCTCTTTCATCACAGACC-3′ R: 5′-AGATGCAGCAAAGCCAAAGT-3’ | BsmI | 196 | TT = 196 GT = 50,146,196 GG = 50,146 |
rs17366743 | T/C | F:5’-TAAGGGAGACATCGGTGAAAC-3′ R: 5′-TTACGCTCCTTCCCCATA −3’ | Bst1107 | 438 | CC = 438 TC = 104.334,438 TT = 104.334 |