Table 3 Forward and reverse primers were designed to target the A/B, XLαs and NESP55 regions
Primers | Primer sequence |
|---|---|
A/B(M)F | 5′-TTCGGCGGGGATATTTAGTC-3′ |
A/B(M)R | 5′-ACAAAAACTCGCTCCAACCG-3′ |
A/B(U)F | 5′-TGGTATTGTGGAGTGGGTTG-3′ |
A/B(U)R | 5′-CCCCACACCAAAACAAAAAC-3’ |
XLαs(M)F | 5′-GTTCGGTTGGGTGTTTTATTTTAC-3’ |
XLαs(M)R | 5′-ATAATTACTCGAACTATTCCCCGAT-3’ |
XLαs(U)F | 5′-GTTTGGTTGGGTGTTTTATTTTATG-3’ |
XLαs(U)R | 5′-ATAATTACTCAAACTATTCCCCAAT-3’ |
NESP55(M)F | 5′-CGTTTTTGTTATTTTTAACGTTCGT-3’ |
NESP55(M)R | 5′-ACAACTCAAAATCTACCTCCTCGTA-3’ |
NESP55(U)F | 5′-TGTTTTTGTTATTTTTAATGTTTGT-3’ |
NESP55(U)R | 5′-ACAACTCAAAATCTACCTCCTCATA-3 |