Fig. 8

ROCK1 partially reversed the regulation of si-circ_0000064 on HG-induced HK-2 cells injury. A WB analysis was used to detect ROCK1 protein expression in HG-induced HK-2 cells transfected with pcDNA3.1 or ROCK1 overexpression vector. B-L HK-2 cells were transfected with si-NC, si-circ_0000064, si-circ_0000064 + pcDNA3.1 or si-circ_0000064 + ROCK1, and then treated with HG. B-C Cell oxidative stress was evaluated by measuring the levels of SOD and MDA. Cell proliferation was detected by CCK8 assay (D) and EDU assay (E). (F-H) The protein expression of PCNA and cyclin D1 was detected using WB analysis. I Cell apoptosis rate was determined using flow cytometry. J-L WB analysis was performed to analyze the protein expression of Bax and Bcl2. *P < 0.05, **P < 0.01, ***P < 0.001