Fig. 7

MiR-532-3p targeted ROCK1. A The binding sites and mutant sites between miR-532-3p and ROCK1 3’UTR were shown. B-C The mRNA and protein expression of ROCK1 in the serum of DN patients with microalbuminuria or macroalbuminuria and normal healthy volunteers was examined by qRT-PCR and WB analysis. D WB analysis was used to measure the protein expression of ROCK1 in HK-2 cells under HG, NG or mannitol condition. E Dual-luciferase reporter assay was used to confirm the interaction between miR-532-3p and ROCK1. F ROCK1 protein expression was detected by WB analysis in HK-2 cells transfected with anti-miR-NC or anti-miR-532-3p. G WB analysis was used to measure ROCK1 protein expression in HK-2 cells transfected with si-NC, si-circ_0000064, si-circ_0000064 + anti-miR-NC or si-circ_0000064 + anti-miR-532-3p. **P < 0.01, ***P < 0.001