Fig. 6

Circ_0000064 regulated HG-induced HK-2 cells injury by sponging miR-532-3p. A QRT-PCR analysis was performed to measure miR-532-3p expression in HG-induced HK-2 cells transfected with anti-miR-NC or anti-miR-532-3p. B-L HK-2 cells were transfected with si-NC, si-circ_0000064, si-circ_0000064 + anti-miR-NC or si-circ_0000064 + anti-miR-532-3p, and then treated with HG. B-C The levels of SOD and MDA were determined to measure cell oxidative stress. Cell proliferation was analyzed using CCK8 assay (D) and EDU assay (E). F-H WB analysis was used to detect the protein expression of PCNA and cyclin D1. I Cell apoptosis rate was analyzed by flow cytometry. J-L The protein expression of Bax and Bcl2 was examined by WB analysis. *P < 0.05, **P < 0.01, ***P < 0.001