Fig. 5From: Circ_0000064 promotes high glucose-induced renal tubular epithelial cells injury to facilitate diabetic nephropathy progression through miR-532-3p/ROCK1 axisMiR-532-3p relieved HG-induced HK-2 cells injury. HK-2 cells were transfected with miR-NC or miR-532-3p mimic and then treated with HG. A The expression of miR-532-3p was detected by qRT-PCR. B-C Cell oxidative stress was analyzed by detecting the levels of SOD and MDA. Cell proliferation was assessed using CCK8 assay (D) and EDU assay (E). F-H The protein expression of PCNA and cyclin D1 was examined using WB analysis. I Cell apoptosis rate was analyzed using flow cytometry. J-L The protein expression of Bax and Bcl2 was determined using WB analysis. **P < 0.01, ***P < 0.001Back to article page