Fig. 4

MiR-532-3p could be sponged by circ_0000064. A The binding sites and mutant sites between circ_0000064 and miR-532-3p were shown. B The expression of miR-532-3p was detected by qRT-PCR in the serum of DN patients with microalbuminuria or macroalbuminuria and normal healthy volunteers. C QRT-PCR was used to measure the miR-532-3p expression in HK-2 cells under HG, NG or mannitol condition. Dual-luciferase reporter assay (D) and RNA pull-down assay (E) were used to assess the interaction between circ_0000064 and miR-532-3p. ***P < 0.001