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Fig. 3 | BMC Endocrine Disorders

Fig. 3

From: Circ_0000064 promotes high glucose-induced renal tubular epithelial cells injury to facilitate diabetic nephropathy progression through miR-532-3p/ROCK1 axis

Fig. 3

Circ_0000064 knockdown alleviated HG-induced HK-2 cells injury. HK-2 cells were transfected with si-NC or si-circ_0000064 and then treated with HG. A QRT-PCR was used to measure circ_0000064 expression. B-C The levels of SOD and MDA were measured to assess cell oxidative stress. CCK8 assay (D) and EDU assay (E) were used to detect cell proliferation. F-H WB analysis was utilized to determine the protein expression of PCNA and cyclin D1. I Flow cytometry was used to assess cell apoptosis rate. J-L The protein expression of Bax and Bcl2 was detected by WB analysis. **P < 0.01, ***P < 0.001

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BMC Endocrine Disorders

ISSN: 1472-6823

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