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Fig. 5 | BMC Endocrine Disorders

Fig. 5

From: Suppression of MIR31HG affects the functional properties of thyroid cancer cells depending on the miR-761/MAPK1 axis

Fig. 5

MiR-761 targets MAPK1. A MAPK1 3’UTR fragment encompassing the putative miR-761 pairing sequence, mature miR-761 sequence, and the mutations in the predicted miR-761 seed region. B and C Effects of miR-761 on the activity of the luciferase reporter construct containing the wild-type (WT) or mutated (MUT) miR-761 pairing site in SW579 and TPC-1 cells. D Relative MAPK1 mRNA expression gauged by qRT-PCR in primary tumor samples and matched normal thyroid samples from the same patients (n = 29), normalized to β-actin expression. E Expression correlation between MAPK1 mRNA and miR-761 in primary tumor samples (n = 29). Correlation value (r) was Pearson’s rank correlation coefficient. F Expression of MAPK1 protein in primary tumor samples and matched normal thyroid samples from the same patients (n = 5) by immunoblotting, with GAPDH as a loading control for expression quantification. G Relative MAPK1 protein expression in SW579, TPC-1, and Nthy-ori 3–1 cells measured by immunoblotting, with GAPDH as a loading control. H Effects of anti-miR-761, anti-miR-NC, miR-761 mimic and miR-NC control on miR-761 expression measured by qRT-PCR, normalized to U6 expression. I Effects of anti-miR-761, anti-miR-NC, miR-761 mimic and miR-NC control on relative MAPK1 protein expression gauged by immunoblotting, with GAPDH as a loading control for expression quantification

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