Fig. 4

MIR31HG function is partly dependent on miR-761. A Effects of the transfection of anti-miR-761 + si-MIR31HG, anti-miR-NC + si-MIR31HG, si-MIR31HG or si-NC on miR-761 expression in SW579 and TPC-1 cells, normalized to U6 expression. B Effects of the indicated transfections on the viability of SW579 and TPC-1 cells by MTS assay. C Effects of the indicated transfections on the proliferation of SW579 and TPC-1 cells, based on the staining with EdU and DAPI. D Effects of the indicated transfections on the apoptosis of SW579 and TPC-1 cells, based on the Annexin V/PI staining. E Transwell assay for the invasion ability of SW579 and TPC-1 cells after the indicated transfections. F Wound-healing assay for the migration rate of SW579 and TPC-1 cells after the indicated transfections. G and H Representative immunoblotting analysis showing the protein levels of Cleaved-caspase-3 and MMP9 in SW579 and TPC-1 cells after the indicated transfections, with GAPDH as a loading control for relative protein quantification. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001