Fig. 3

MIR31HG regulates miR-761 expression by directly pairing to miR-761. A MIR31HG segment harboring the putative miR-761 pairing region, mature miR-761 sequence, and the mutations in the predicted miR-761 pairing site. B and C Effects of miR-761 on the activity of the luciferase reporter construct containing the wild-type (WT) or mutated (MUT) miR-761 pairing site in SW579 and TPC-1 cells. D Relative miR-761 expression as gauged by qRT-PCR in primary tumor samples and matched normal thyroid samples from the same patients (n = 29), normalized to U6 expression. E Expression correlation between miR-761 and MIR31HG in primary tumor samples (n = 29). Correlation value (r) was Pearson’s rank correlation coefficient. F Relative miR-761 expression in SW579, TPC-1, and Nthy-ori 3–1 cells measured by qRT-PCR, normalized to U6 expression. G Effects of MIR31HG expressing plasmid introduction and pcDNA control transfection on MIR31HG expression in SW579 and TPC-1 cells by qRT-PCR, normalized to β-actin expression. H Effects of the transfection of si-MIR31HG, si-NC, MIR31HG expressing plasmid, and pcDNA control plasmid on miR-761 expression measured by qRT-PCR, normalized to U6 expression. ***P < 0.001, ****P < 0.0001