Table 2 Primer sets used for qRT-PCR assay
Gene | Primers | Primer sequence | Tm |
|---|---|---|---|
RIP1K | Forward Reverse | 5′ –′5′-GCC TTG AAC TTC TGA CCT CA- 3′ 5′- AGC ATC CAC TGA TTG GAC CT − 3′ | 58 |
RIP3K | Forward Reverse | 5’- AGT CTT CCA GAT GGT GGA GA - 3′ 5′- AGC ATC CAC TGA TTG GAC CT - 3′ | 58 |
MLKL | Forward Reverse | 5’- CTG TTA CTT CAG GTT GAG CA - 3′ 5′- GGC AAG GAG ACA GAA CTC AA - 3′ | 59 |
Beta-Actin | Forward Reverse | 5-GAT CTC CTT CTG CAT CCT GT-3′ 5′-TGG GCA TCC ACG AAA CTA C- 3′ | 57 |