Fig. 2

GH3 cells are resistant to bromocriptine and MMQ cells are sensitive to bromocriptine. a, b Western Blot analysis of the DRD2 protein expression level of different concentrations of bromocriptine (12.5 μM, 25 μM, 50 μM, 100 μM) treated MMQ cells for 48 h.^**P < 0.01 vs. 0 μM,^***P < 0.001 vs. 0 μM, (n = 3). c RT-qPCR analysis of DRD2 mRNA expression levels in MMQ cells treated with different concentrations of bromocriptine(12.5 μM, 25 μM, 50 μM, 100 μM) for 48 h.^**P < 0.01 vs. 0 μM,^***P < 0.001 vs. 0 μM, (n = 3). d CCK-8 kit detects the cell viability of bromocriptine on MMQ and GH3 cells. e, f The expression of PRL protein in the supernatant of GH3 and MMQ cells treated with bromocriptine for 48 h was detected by ELISA.^*p < 0.05 vs. 0 μM,^***p < 0.001 vs. 0 μM,^****p < 0.0001 vs. 0 μM, (n = 6)