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Fig. 5 | BMC Endocrine Disorders

Fig. 5

From: LncRNA HCP5 knockdown inhibits high glucose-induced excessive proliferation, fibrosis and inflammation of human glomerular mesangial cells by regulating the miR-93-5p/HMGA2 axis

Fig. 5

MiR-93-5p inhibition reversed the effects of HCP5 knockdown. A The availability of miR-93-5p inhibitor was checked using QPCR. In the si-NC, si-HCP5, si-HCP5 + anti-NC and si-HCP5 + anti-NC groups, (B) cell proliferation was checked using CCK-8 assay. C and D Cell apoptosis was analyzed by flow cytometry assay. E The levels of Cleaved-PARP and Cleaved-caspase 3 were quantified using western blot. F The protein levels of FN, Col IV and Col I were detected by western blot. G The release of TNF-α, IL-6 and IL-1β was determined using ELISA kits. **P < 0.01 and ***P < 0.001

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