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Fig. 3 | BMC Endocrine Disorders

Fig. 3

From: A novel mutation of WFS1 gene leading to increase ER stress and cell apoptosis is associated an autosomal dominant form of Wolfram syndrome type 1

Fig. 3

Luciferase reporter assays in HEK293T cells. a Sanger sequencing was used to verify the sequence of mutant (c. 2067_2076 del10, p.W690fsX706) WFS1 and mutant (c. 2648_2651 del4, p.F883fsX950) WFS1 expression plasmids. b HEK293T cells were transfected with the ERSE reporter vector together with the control (pcDNA3.1), wild type WFS1 (WT), mutant p.W690fsX706 (p.W690X) WFS1, or the positive control mutant p.F883fsX950 (p.F883X) WFS1 expression plasmid. Cells were treated with or without thapsigargin (TG, 10 nmol/L) for 6 h and the relative luciferase intensity was measured. The results were normalized to Renilla reniformis luciferase activity. Data were presented as mean ± SEM. One-way ANOVA, Tukey’s post-hoc test, **P < 0.01 and ***P < 0.001

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BMC Endocrine Disorders

ISSN: 1472-6823

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