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Table 1 Primers for amplifications of IL-6-174G/C and IL-10-(-1082) by polymerase chain reaction, 2018

From: Association of IL-10 (− 1082 A/G) and IL-6 (− 174 G/C) gene polymorphism with type 2 diabetes mellitus in Ethiopia population

Genetic polymorphism

Primer sequence

Annealing C0

Amplicon size

IL-6-174G/C

Forward primer

 

GG-180 bp,

GC-204 and 180 bp

CC-204 bp

5′-ACTTTTCCCCCTAGTTGTTTCTTTC-3’

60

Reverse primer

5′-AGACATGAGCCTCAGACATCTCAGT-3’

IL-10-(−1082)

Sense IL-10-(− 1082) G

  

5′-CTACTAAGGCTTCTTTGGGAG-3’

56

161

Sense IL-10-(−1082) A

 

5′-ACTACTAAGGCTTCTTTGGGAA-3’

161

Antisense IL-10-(−1082)

 

5′-CAGTGCCAACTGAGAATTTGG-3′.

 
  1. Footnote: bp base pair, TM Temperature.