Fig. 4From: Thyroid-stimulating hormone decreases the risk of osteoporosis by regulating osteoblast proliferation and differentiationEffect of TSH on the mRNA expression of ALP, COI1, BMP2 and Runx2. The mRNA expression of ALP (a), COI1 (b), BMP2 (c) and Runx2 (d) was determined by real-time RT-PCR. ALP (e) and BMP2 (f) mRNA levels were detected at 0, 12, 24 and 48 h of 100 mU/mL TSH treatment. The data are expressed as the means±SDs. *P < 0.05, **P < 0.01, ***P < 0.001 versus the control group. As shown by western blot analysis, TSH (10 and 100 mU/mL) promoted Runx2 and osterix protein expression (g)Back to article page