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Fig. 2 | BMC Endocrine Disorders

Fig. 2

From: Acacetin ameliorates insulin resistance in obesity mice through regulating Treg/Th17 balance via MiR-23b-3p/NEU1 Axis

Fig. 2

NEU1 was the target of miR-23b-3p, and miR-23b-3p upregulation further enhanced the effects of obesity on NEU1 expression. a Sequences of NEU1-WT (top), miR-23b-3p (middle) and NEU1-MUT (below) were listed. b Dual-luciferase reporter assay confirmed that NEU1 was the target of miR-23b-3p. c-d Relative NEU1 protein/GAPDH expressions after obesity-induced IR model construction and miR-23b-3p upregulation were measured with Western blot. GAPDH was employed as internal control. All experiments have been performed in triplicate and data were expressed as mean ± standard deviation (SD). †††P < 0.001, vs. Control; ***P < 0.001, vs. Control; ^^^P < 0.001, vs. Model; ###P < 0.001, vs. Acacetin+MC. WT: wild-type; MUT: mutated; M: mimic; NEU1: Neuraminidase 1

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