Fig. 1

MiR-23b-3p upregulation promoted the effects of obesity-induced IR of mice. a Body weight of mice after obesity-induced IR model construction, Acacetin injection and miR-23b-3p upregulation was measured. b Fat percent of mice was calculated after obesity-induced IR model construction, acacetin injection and miR-23b-3p upregulation. c-d Levels of fasting blood glucose (c) and fasting insulin (d) after obesity-induced IR model construction, acacetin injection and miR-23b-3p upregulation was determined. e-f AUC of mice for OGTT (e) and ITT (f) after obesity-induced IR model construction, acacetin injection and miR-23b-3p upregulation were quantified. g-j Levels of inflammatory cytokines CRP (g), IL-6 (h), TNF-α (i) and MCP1 (j) after obesity-induced IR model construction, acacetin injection and miR-23b-3p upregulation were detected with ELISA. k Relative expressions of IL-17 and Foxp3 after obesity-induced IR model construction, acacetin injection and miR-23b-3p upregulation were determined using qRT-PCR. GAPDH was used as internal control. All experiments have been performed in triplicate and data were expressed as mean ± standard deviation (SD). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, vs. Control; ^{^}P < 0.05, ^{^^}P < 0.01, ^{^^^}P < 0.001, vs. Model; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, vs. Acacetin+mimic control (MC). miR-23b-3p: MicroRNA-23b-3p; AUC: Area under the Curve; OGTT: Oral glucose tolerance test; ITT: Insulin tolerance test; CRP: C-reactive protein; IL-6: Interleukin-6; TNF-α: tumor necrosis factor-α; MCP1: monocyte chemoattractant protein 1; ELISA: enzyme-linked immunosorbent assay; Foxp3: Forkhead Box P3; qRT-PCR: quantitative real-time polymerase chain reaction