Table 1 Sequences of primers used to amplify related genotypes, restriction enzymes for digestion, and sizes of PCR products after digestion

IGF-1R ^a

5’-TGCTTTAATTACGGTTTCTTC-3’

60 °C

Mnl I

G:132, 77, 50, 20 bp

5’-GCTTTTCAGGAACTTTCTCTT-3’

A:132, 97, 50 bp

IGF-2

 + 3123^a

5’-CCCCAGGTCACCCCATGTGA-3’

65 °C

ApaI

G: 173, 63 bp

5’-GGGCTGGAGGCAGCTGAGTG −3’

A: 236 bp

 + 3580^a

5’-CCACCCCTTCTGGGAAGCTAAAAG-3’

56 °C

MspI

A: 122, 118 bp

5’-CCCTCGGTCCTCCAGGAATGGACA-3’

G: 122, 118, 34 bp

IGF-2R ^a

5’-AACAATGGTTAAAGCCGGATTG-3’

67 °C

Nci I

A: 456 bp

5’-GGCCCGGGTGCAGCCAGGCACTG-3’

G: 307, 149 bp

INS ^a

5’-GGGTCCCCTGCAGAAGCGTGGCA-3’

65 °C

PstI

T: 562 bp

5’-CTCCCTCCACAGGGACTCCATC-3’

C: 470, 92 bp

IGF-1

 + 1770^b

5′-tagaatattatttatagtattaaac

[a/g]aggttttactagatatgtagtaact −3’

60 °C

−

T:VIC dye

C:FAM dye

 + 6093^b

5′-acagataaaagatgtaagtagacag

[c/t]ttgaggtttcagagtccctcctgc − 3’

60 °C

−

G: VIC dye

A: FAM dye

 -202^b

5′-tcgcccgggcacctgctcctcgtgc

[g/t]cacgcccggagcccgggtcaccttg

60 °C

−

C: VIC dye

A: FAM dye