Table 1 Sequences of PCR primers
Gene | Primer | Sequence(5′_3’) | Accession no. |
|---|---|---|---|
GnRH | GnRH-F | TCCAGCCAGCACTGGGTCCTA | NM_012767.2 |
GnRH-R | GGGTTCTGCCATTTGATCCTC | ||
β-actin | β-actin-F β-actin-R | GGAGATTACTGCCCTGGCTCCTA GACTCATCGTACTCCTGCTTGCTG | NM_017008.4 |
Gene | Primer | Sequence(5′_3’) | Accession no. |
|---|---|---|---|
GnRH | GnRH-F | TCCAGCCAGCACTGGGTCCTA | NM_012767.2 |
GnRH-R | GGGTTCTGCCATTTGATCCTC | ||
β-actin | β-actin-F β-actin-R | GGAGATTACTGCCCTGGCTCCTA GACTCATCGTACTCCTGCTTGCTG | NM_017008.4 |