Fig. 2

Frozen sections of placental tissues from the patient and a gestational age-matched control subject were fluorescent-immunohistochemically stained for vasopressinase (a) or vasopressinase and GLUT4 (b) with DAPI. c: Membrane or cytosolic fractions of samples were extracted from the placental tissues of the patient and the gestational age-matched control subject, and subjected to western blotting for GLUT4, vasopressinase (identical to IRAP), Akt or phosphor-Akt (ser473)